Acute exercise modulates BDNF and pro-BDNF protein content in immune cells

Purpose. Although several studies have shown that immune cells stimulated by in vitro stress
are capable to produce neurotrophins (NTs), there is still no evidence whether physiological
stress, such as exercise, can modulate the in vivo levels of brain-derived neurotrophic factor
(BDNF) in peripheral blood mononuclear cells (PBMCs).
Methods. This work investigated whether acute exercise modulates the expression of BDNF,
pro-BDNF and p75NTR in the PBMCs of ten healthy young men who performed a cycling
incremental test to exhaustion (MAX) or exercised at individual anaerobic threshold (IAT).
The PBMCs expression of stress response proteins and the level of circulating BDNF, VEGF,
PDGF-BB, bFGF, pro- and anti-inflammatory cytokines were analyzed as well.
Results. A major finding is that both sessions of acute exercise regulated the content of
BDNF isoforms within PBMCs in a manner related to the physiological stress exerted. While
the pro-BDNF increased after both MAX and IAT protocols, BDNF showed a kinetics
dependent on exercise type: MAX induced a 54% protein increase immediately after
exercise, followed by a significant drop 60 minutes after its conclusion (38% lower than
baseline). Differently, in the IAT, BDNF increased significantly up to 75% from baseline
throughout the recovery phase. All physiological parameters, as well as the p75NTR receptor
and the stress inducible proteins were also differently regulated by the two exercise
conditions.
Conclusions. These data supported the hypothesis that PBMCs might produce and secrete
BDNF isoforms, as well as modulate the proteins p75NTR, Bcl-xL, hsp90, hsp27 and αBcrystallin,
as part of the physiological stress response induced by acute exercise, offering a
novel example of bidirectional interaction between nervous and immune systems.